Enhancing the Efficacy of Antibiotics Against Pseudomonas aeruginosa Using Nanoemulsions of Myrtle Plant Extract
Abstract
Biofilms made by Pseudomonas aeruginosa create a shield that helps the bacteria resist antibiotics and the body's defenses, often leading to long-lasting and hard-to-treat infections. This study looked at how effective nanoemulsions made with Myrtle plant extract could be in making regular antibiotics work better against P. A focus on stopping the growth of biofilms in Pseudomonas aeruginosa and changing the activity of genes related to biofilms (pelA and pslA). Nanoemulsions with Myrtle extract were made and mixed with low amounts of gentamicin and imipenem. They tested how well it worked against biofilms using the crystal violet test and counted the number of colonies. In addition, we looked at how important genes related to biofilm (pelA and pslA) were affected by using a test called real-time quantitative polymerase chain reaction (RT-qPCR). The combination treatments worked really well to lower P. The formation of biofilm by aeruginosa ATCC 27853 is significantly higher when compared to using antibiotics alone (P < 0. 0001) The CV assay results showed that the biofilm mass went down by more than 75%, and the CFU counting revealed a significant drop in the number of living bacteria. The RT-qPCR tests showed that adding Myrtle-based nanoemulsions with antibiotics greatly reduced the activity of the pelA and pslA genes (P < 0. 05) These results indicate that tiny drops of Myrtle extract can make regular antibiotics work better by breaking down biofilms and reducing the activity of genes related to biofilms in P. A problem with bacteria called aeruginosa is being addressed with a hopeful approach to fight infections that don't respond to regular treatments.
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